Monarch butterflies are among the most popular insect species in the world and are an icon for conservation groups and environmental education programs. Monarch caterpillars and adults are easily recognizable as welcome visitors to gardens in North America and beyond, and their spectacular migration in eastern North America (from breeding locations in Canada and the United States to overwintering sites in Mexico) has captured the imagination of the public. Monarch migration, behavior, and chemical ecology have been studied for decades. Yet many aspects of monarch biology have come to light in the past few years. These aspects include questions regarding large-scale trends in monarch population sizes, monarch interactions with pathogens and insect predators, monarch molecular genetics, and large-scale evolution.